Plasmid

Part:BBa_K3505011:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)


pDGB1 omega 2 (Ω2) for Bacterial GoldenBraid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3277
    Illegal PstI site found at 3316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3277
    Illegal BamHI site found at 3298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3277
    Illegal XbaI site found at 3304
    Illegal PstI site found at 3316
    Illegal NgoMIV site found at 948
    Illegal NgoMIV site found at 1350
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2918
    Illegal BsaI.rc site found at 6


Design Notes